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The main things the reader needs to take away at this point are the following: Free cortisol rises and falls across 24-hours in a predictable pattern Loss of this variation can be a serious problem There are key features to the pattern that include an overnight rise in free cortisol and an additional surge in free cortisol after waking The profiles need to be interpreted in the light of how the patient feels throughout the day and night

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However, despite markedly different time courses, a consistent and selective increase in N3 was observed in all studies

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