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Available online at: www.ejabf.journals.ekb.eg (Accessed March 2, 2025)

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The enzyme activity of histone deacetylase 8 is modulated by a redox-switch

Using this configuration, the instrument settings were optimized, and the emission data were collected using a 512/25-nm band-pass emission filter (green channel)

The lysate was clarified by centrifugation at 15,000 g for 15 minutes, protein concentrations were determined using the BCA total protein assay, and then appropriate volumes of the lysates were diluted with 2 reducing Laemmli buffer and heated for ten minutes at 95 C