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coli recombinant strains were taken from the induced cultures and adjusted to OD 600 of 1.0 in fresh LB medium, and serial dilutions of these adjusted sample cultures were plated onto non-selective LB and LB agar plates with kanamycin, streptomycin, or chloramphenicol, and incubated at 37 C (or 30 C for plasmids with the thermo-sensitive pSC101 replicon) until colonies were visible

We extend our previous work, which identified changes in vision-based optomotor responses in larval zebrafish 1-week and 2-weeks after developmental exposure to BPA 42 , to ask if transient, developmental exposure to BPA causes persistent effects in retina that can be measured in adulthood (3-4 months postexposure)

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Introduction to NF-B: players, pathways, perspectives

Data are representative of or pooled from two independent experiments ( a , b ) or representative of four tonsil specimens ( c , d )