Extensive experimental and computational studies have shown that the binding of GSH to GSTs decreases the p K a of the GSH thiol group from around 9 to about 6 in the active site through hydrogen bonding and salt bridges and through the positive electrostatic potential of the G-site 41,42
AMP-activated protein kinase and human cancer: cancer metabolism revisited
As seen in Supplemental Figure 4B, the sum of total intracellular hydroperoxy- and hydroxy-phospholipids following IL-13 stimulation was approximately 2 nmol/mg protein, that is, sufficient amounts to quantifiably impact the change in intracellular GSH (and the increases in GSSG) under these two conditions, as shown in Supplemental Figure 4C under similar conditions
Proc Natl Acad Sci U S A 81:935939 Roberts CT Jr, Lasky SR, Lowe WL Jr, Seaman WT, LeRoith D (1987) Molecular cloning of rat insulin-like growth factor I complementary deoxyribonucleic acids: differential messenger ribonucleic acid processing and regulation by growth hormone in extrahepatic tissues
L- 2-
Safety animal and some human data profiles generally look good, but clinical fertility trials are sparse