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Briefly, 5 g of importin or importin complexes were incubated on ice for one hour, with increasing molar excesses (typically between 0.5 and 10) of Ran-GTP (or Ran-GDP), RanBP1, and/or IBB

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It has an excellent safety profile, which is why higher doses like 1000 mcg are common and well-tolerated

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Compromising ATG5 expression in LEC did not affect other histone modifications, such as H3K27 acetylation or H3K4me3 methylation (Supplementary Fig