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Worthington 1997

Following vortexing and passage through a 70 m strainer, the suspension of IECs was washed twice by centrifugation at 600 g for 3 min and collected for subsequent experiments

Protein concentrations were adjusted to 2 mg/mL and samples (100 l, 200 g total proteins/sample) were added to new eppendorf tubes containing pre-weighed urea (48 mg/sample)

It should be noted that the toxic effects of AF occurred in the absence of exogenous oxidative stress (e.g., no added H 2 O 2 or other oxidant) on the worms

This range is typically sufficient for healthy individuals who want to provide their body with a bit of extra insurance against everyday oxidative stress