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Double phosphorylation of the activation loop of MAPKs was confirmed in western blots with phosphoMAPK-specific antibody and/or mass spectrometry

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Only recently, attention has been paid to the study of metal ions and inorganic small molecules to intervene in the mechanism of physiopathological processes, with special attention to the differences between inorganic and organic drugs in terms of absorption, transit, distribution, metabolism, toxicology, and pharmacological effects (Meiling et al., 2013)