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After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes

Human safety data is limited, and individual results may vary

therefore, its complete replacement must be recognized as a key point within the 3Rs framework [5]

The colonisation of BCBM cells includes a series of steps, including reawakening from dormancy state, vascular co-option for micrometastases formation, and metabolic reprogramming

This buildup is thought to trigger downstream effects, including the formation of tau-containing neurofibrillary tangles, due to an imbalance between A production and clearance

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