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The transduced MAP cells were plated on 75 g/ml hygromycin-7H11 plates and incubated at 37C for 4-6 weeks to select colonies that had successfully accomplished homologous recombination ( Figure 1A )

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doi: 10.1371/JOURNAL.PONE.0050568 19

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Likewise, along with previous beneficial effect of BPC 157 in gastrointestinal tract, and inflammatory bowel disease complications that were accordingly solved (10, 11), poor healing of the fistulas (35), unhealed intestinal anastomosis (42, 43), massive intestinal resection leading to short bowel (43)) this study shows BPC 157's high potential to heal colon-colon anastomosis along with cysteamine colitis, thus, potential to counteract the consequences of the anastomosis for cysteamine colitis, and vice versa, convalescing the increased healing failure of anastomosis healing due to concomitant presentation of severe colitis