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Quantitative real-time RT-PCR The total RNA extraction, cDNA synthesis, and real-time PCR were performed as described previously [13]

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Ruan G-Y, Ye L-X, Lin J-S, Lin H-Y, Yu L-R, Wang C-Y, Mao X-D, Zhang S-H, Sun P-M (2023) An integrated approach of network pharmacology, molecular docking, and experimental verification uncovers kaempferol as the effective modulator of HSD17B1 for treatment of endometrial cancer

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3) TNF- inhibitor (TNFi) TNF- triggers inflammatory responses and is produced by activated monocytes, macrophages, and T lymphocytes