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2247-C RACIAL DISPARITIES IN PREVALENCE AND INCIDENCE OF CIRRHOSIS AND EXTRAHEPATIC MANIFESTATIONS IN NONALCOHOLIC FATTY LIVER DISEASE Majd Bassam Aboona 1 , Claire Faulkner 1 , Pooja Rangan 2,3 , Vincent Chen 4 , Cheng Han Ng 5 , Daniel Q Huang 6 , Mark Dhinesh Muthiah 6 , Donghee Kim 7 , Moises Ilan Nevah Rubin 2,3 , Ma Ai Thanda Han 2,3 , Michael Fallon 2,3 and Karn Wijarnpreecha 2,3 , (1)University of Arizona College of Medicine - Phoenix, Phoenix, AZ, (2)Banner University Medical Center, (3)University of Arizona College of Medicine Phoenix, Phoenix, AZ, (4)University of Michigan Medical Center, (5)Yong Loo Lin School of Medicine, National University of Singapore, (6)National University Health System (NUHS), (7)Stanford University Medical Center Background: Nonalcoholic fatty liver disease (NAFLD) has increased in prevalence in recent years

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ML was supported by BMBF, grant 01GM2202A

The t-BuOOH-induced human keratinocyte death could be suppressed by ferroptosis blockers (ferrostatin-1, liproxstatin-1), lipid antioxidant -tocopherol, and the iron chelator deferoxamine (Wenz et al., 2018)

Extracellular vesicle isolation Extracellular vesicles from ascitic fluid samples were purified by differential centrifugation as described previously 129 with some modifications