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12-plex RNA-ISH exhibited sufficient sensitivity and specificity for some high-expressing cell lineage markers, but struggled to adequately measure genes expressed at lower levels, including ITGA4, ITGB7 , MADCAM1 , and FLT3 (Supplementary Fig 13a, b, Supplementary Table 3)

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Approximately 1 10 6 T241-vector or -FGF-2 tumor cells were subcutaneously injected into the back along the mid dorsal line of each C57BL/6 mouse

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