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For flow cytometry, cells were seeded at 1 10 5 per well in a 12-well plate with medium supplemented with 2% FBS during 24 h before being treated as indicated

doi: 10.1002/14651858.CD001301.pub2 224 ArhinSKZhaoYLuXChetryMLuJ

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The outcomes demonstrated that DON boosted gene expression of cellular mitochondrial autophagy marker proteins LC3 and P62, a crucial indicator of autophagy induction, and produced ROS buildup in pig spleen cells (86)

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