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Niacinamide increases serotonin precursors, benefiting mood disorders

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LCMS metabolomics analysis Sample preparation HCT116s cells (1 10 5 ) treated with or without 5 M 5FU were cultured in DMEM for 24 h and 72 h or supplemented with media containing uniformly labelled 13 C 6 -glucose (25 mM) for 6 h before sampling

Treatment Schedule: Injections are typically administered three times per week, with at least one day in between each injection to ensure optimal results

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