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Apocynin, a NADPH oxidase (NOX) inhibitor, effectively reduced ROS and increased NO levels in endothelial (HUVEC) and hypertensive rat models, improving vascular reactivity [149]

For experiments in 6-well plates measuring relative cell numbers at indicated time points, the medium was aspirated, washed with PBS, and 500 L of 0.25% trypsin (Thermo Scientific 25200056) was added to wells for 20 minutes

Nagy Z, et al

The ORR of 6% for Parts 1 and 2 combined was below the pre-specified threshold for efficacy (15%)

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However, with the growing evidence, it was shown that SCFAs are important in regulating cardiovascular functions 67