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A small number of NSCs remain in adults even if most of them are quiescent (recently reviewed in Zhao and Moore, 2018)

In this study, iPSCs have been differentiated using DMEM/F12 medium supplemented with L-glutamin, heparin, and B27 for astrocytes or vasculife basal medium supplemented with FGF, acid ascorbic, hydrocortisone, glutamine, and growth factor for brain endothelial cells