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Nucleic Acids Res 48 , 28532865 (2020)

Western blot analyses were performed on nitrocellulose membranes after blocking in TBS-T containing 5% milk, using the anti-BAX antibody (clone 2D2, Cell Signaling Technologies, 1:1000) and the anti-cytochrome c antibody (clone 7H8.2C12, Abcam, 1:1000 dilution, ab13575) and incubating overnight at 4 C

This adjustment is justified by the evidence that these mutations, typically associated with AML evolving from an antecedent hematologic disease, can also be detected in de novo AML and confer adverse risk even in the absence of MR morphological features and/or a prior history of MDS

These studies suggest that ferroptosis occurs in CKD, as GPX4 is a primary regulator of ferroptosis, and this can lead to the release of large amounts of lipid peroxides, potentially causing changes in the phenotype of tubular epithelial cells (16, 23, 24)

2, Supplementary Figs

Timing and Dosing Effects of Exosome Exposure on the Growth Rate of Nave Monocytes, Emily M