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[Products in use] Extracellular LDH : Cytotoxicity LDH Assay Kit-WST (Product code: CK12) Phosphatidylserine: Annexin V Apoptosis Plate Assay KitProduct code: AD12 Cell viability : Cell Counting Kit-8 (Product code: CK04) Intracellular Fe 2+ : FerroOrange (Product cose: F374) *Normalized with Hoechst 33342 fluorescence intensity Lipid peroxidation : Lipid Peroxidation Probe -BDP 581/591 C11- (Product code: L267) [Experimental conditions] Cell type: HepG2 cell(210 4 cells/well) Drugs: Staurosporin(5 mol/l), Erastin(25 mol/l), Erastin+RSL3(both 25 mol/l) *Diluted in serum-free medium

Glutathione is important in the antioxidant defense, nutrient metabolism, and regulation of cellular events including gene expression, DNA and protein synthesis, cell proliferation and apoptosis, signal transduction, cytokine production and protein glutathionylation ( de novo reduced glutathione ( Because cysteine contains sulfur, cysteine supports sulfur-dependent metabolism

These modular domains ensure that each JMJ demethylase acts in a context-appropriate manner

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