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After being washed with 0.01 M PBS, the cells were counterstained with DAPI for 10 min and imaged with an Olympus BX63 microscope (Olympus, Japan)

Similarly, the protein concentration of Ppar in fish fed the HFD+B2 diet was significantly higher than fish fed HFD, while the opposite was the case for Srebp-1 where, compared to fish fed HFD, fish fed the HFD+B2 diet showed lower hepatic Srebp-1 protein concentration ( Figure 6C )