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When it is secondary to an enzymatic block that is proximal in the pathways of cobalamin reduction or lysosomal efflux, it is also associated with homocystinuria because of impaired production of methylcobalamin

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In a 48-well plate, 250 l diluted cells (50,000 cells/well) were combined with 125 l of the transfection complex mixture and incubated for 24 h before removing RNP medium and replacing with DMEM with 10% FBS and 100 units/ml penicillin/streptomycin (15140122

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Acta Histochem 113:656662 Kazak F, Yarim GF (2017) Neuroprotective effects of acetyl-l-carnitine on lipopolysaccharide-induced neuroinflammation in mice: involvement of brain-derived neurotrophic factor