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2.2 Primary culture of myocytes and thermal stress trial 2.2.1 Primary culture of myocytes Juvenile fish, each weighing approximately 5 g, were dissected under sterile conditions to obtain muscle tissue, which was then cut into pieces and transferred to a 24-well cell culture plate

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For the survival study, animals were treated with i.p

EdU staining Cells subjected to genetic treatments were seeded into 12-well plates at a density of 5 10 cells per well and cultured for 24 hours

Brain Res Bull 116:98105

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