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Add solvent slowly down the vial wall, never directly onto the powder, and mix gently by swirling

Tissue culture flasks, tissue culture dishes ( = 60 mm), 24-well plates, 96-well plates, Tryptic soy agar (TSA) plates, and Mueller-Hinton (MH) broth were obtained from Becton Dickinson and Company (Franklin Lakes, NJ), respectively