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For passage 2 cultures in which clear escape was observed, as evidenced by the appearance of numerous GFP-positive cells, RNA was isolated from aliquots of supernatant containing selected viral populations using NucleoSpin 96 Virus Core Kit (Macherey-Nagel)

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Disclosure of interest: Khudayarova S.M.:nothing to disclose, Rakhmatullayeva G.K.: nothing to disclose E-Posters - RISK FACTORS - PRIMARY AND SECONDAR PREVENTION 05.00 - RISK FACTORS AND PREVENTION (PRIMARY AND SECONDARY) - 05.02 - SECONDARY PREVENTION P2555 - ESOC25-2084 AN EVALUATION OF MEDICAL MANAGEMENT FOR SECONDARY PREVENTION OF ISCHAEMIC STROKE IN A TERTIARY STROKE CENTRE Nicole Cosgrave 1,2 , Basmah Karembaks 1 , Vanessa Farnan 1,2 , Anne-Marie Liddy 2 , Rory Durcan 2 , Karl Boyle 2 , David Williams 1,2 1 Department of Medicine, RCSI University of Medicine and Health Sciences, Dublin, Ireland, 2 Department of Geriatrics and Stroke Medicine, Beaumont Hospital, Dublin, Ireland Background and Aims: Recurrent ischaemic strokes occur in 9-15% of patients within one year

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Double cleanse using the Im Fabulous Cosmetics Organic Luxe Cleanser to remove impurities without disrupting the acid mantle