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Zhang K, Li S, Zhao Q et al (2022) Multi-Omics Profiling Reveals Se Deficiency-Induced Redox Imbalance, Metabolic Reprogramming, and Inflammation in Pig Muscle

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Immunofluorescence Staining and Confocal Imaging Enteroid monolayers were fixed for 40 min in 4% paraformaldehyde (Electron Microscopy Sciences, United States), washed with PBS for 10 min, permeabilized and blocked for 1 h with PBS containing 15% fetal bovine serum, 2% BSA, and 0.1% saponin, all at room temperature (RT)