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Lower urinary tract symptoms are a common non-motor symptom in individuals with PD and atypical parkinsonian disorders and are divided into disturbances of the storage phase, mostly due to bladder detrusor hyperactivity, and disturbances of the voiding phase due to detrusor-sphincter dyssynergia or hypocontractile detrusor [1, 88, 167]

The same has been observed in brain scans of those with self-reported sleep deprivation

Transfection of cells, Immunofluorescence microscopy and Subcellular fractionation of transfected cells For subcellular fractionation, HEK293T cells originating from ATCC (CRL-3216) were transfected using Ca-phosphate precipitation method