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Amino acids V12 (putative oxyanion hole), F112 (active site architecture), N108 (flavonoid binding) and C13 (GSH stabilization) were fully randomized by site saturation mutagenesis (NNK codons), and the created Pt GSTF8 variants were tested for conversion of flavan-3,3,4-triol ( 4 ) to cyanidin ( 8 ), and for their GSH-transferase activity towards CDNB ( 5 ) as a control of catalytic competence

A.ChelmowD.DaveyD

Diabetes mellitus is one of the major risk factor for CAD and other risk factors for CAD are hypertension, obesity, smoking and dyslipoproteinemias [2]

& Capecchi, M

These results confirmed that ROS and the excitatory potency of acidic amino acids such as glutamate are involved in methylmercury-induced neurotoxicity in cerebral neuron cultures