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Adding the water-soluble glutathione tag marks these compounds for further processing through the mercapturic acid pathway and eventual excretion

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NAC can inhibit ferroptosis by replenishing intracellular glutathione and supporting GPX4 activity, but its broad mechanism of action makes it difficult to attribute benefits specifically to ferroptosis inhibition ( Key hurdles for clinical translation of ferroptosis inhibitors include ( 5.2 Gut microbial modulators Understanding the role of gut microbiota in ferroptosis opens up exciting possibilities for new therapeutic strategies

Treatment with inactive, negative control compounds ( 11 and 12 ), structurally related to the active inhibitors 1 - 10 , had minimal effect on the GSH/GSSG in treated worms

Step 3: Add water to the peptide vial