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However, we should keep in mind that vitamin B12 deficiency can be present with either low or high serum levels of the vitamin, as the later ones can arise from impaired tissue uptake and activity at cellular level and it is irrelevant to establish direct causation

Cells were then incubated for 30 minutes at RT

MOTS-c has a short half-life (46 hours), so missing a single dose doesn't create long-term metabolic disruption, but it does interrupt the AMPK activation pattern for that 48-hour window

This prevents confusion later when you are pulling doses at different points in the vial's life

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Cross-reactive T cells T cells are inherently more cross-reactive than B cells because they recognize short peptide sequences presented on the major histocompatibility complex (MHC) rather than complex structural epitopes and because they can target conserved internal proteins rather than just surface and structural proteins that are accessible to antibodies on extracellular virus