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PVDF membranes were blocked in 5% (w/v) milk powder in PBST (140 mM NaCl, 2.7 mM KCl, 10.1 mM Na 2 HPO4, 1.8 mM KH 2 PO4, 0.1% (v/v) Tween-20) or TBST (150 mM NaCl, 20 mM Tris/HCl, pH 7.5, 0.1 % (v/v) Tween-20) for 1 h at room temperature

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Tempesta E, Casella L, Pirrongelli C, Janiri L, Calvani M, Ancona L

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For this purpose, the allicin sample was dissolved in methanol-D4, and the 1H spectrum was recorded at 298 K using a Bruker Avance III 500 spectrometer

Vitamin B-12 supplements also are recommended for women who are pregnant or breastfeeding exclusively and follow vegetarian or vegan diets