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Meanwhile, a culture of EcN pSIM18 was grown to mid-log phase at 30 C in LB + 200 g/mL hygromycin, then expression of the lambda Red proteins was induced by incubating the cells at 42 C for 15 min, followed by 15 min on ice

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The same potent growth-promoting effects that make it attractive also create real risks that demand respect

Predrag Sikiric, Sven Seiwerth, Rudolf Rucman, Branko Turkovic, Dinko Stancic Rokotov, Luka Brcic,Marko Sever, Robert Klicek, Bozo Radic, Domagoj Drmic, Spomenko Ilic, Danijela Kolenc, Gorana Aralica, Mirjana Stupnisek, Jelena Suran, Ivan Barisic, Senka Dzidic, Hrvoje Vrcic, Bozidar Sebecic

10.1093/omcr/omw006 104