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The proportion of cells with GFP-tagged Atg8 protein forming puncta and RFP-tagged Atg8 protein entering the vacuole was determined by counting these cells among at least 200 yeast cells per condition

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The primary outcomes were in-hospital mortality, death within 30 days of admission, and ICU admission

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MOTS-c activates the AMPK pathway, which is the same pathway triggered by exercise and caloric restriction