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At the end of fixation cells were washed with Ca 2+ /Mg 2+ PBS and treated with monoclonal rabbit antibodies against N-terminal extracellular domain of amyloid precursor protein (dilution in PBS to the concentration 3.84 g/mL, Abcam Limited, Discovery Drive, Cambridge Biomedical Campus, Cambridge, United Kingdom, ab126732) at 4 C overnight

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GT was actively involved in drafting the manuscript