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To explore the possibility of a direct protein-protein interaction between Ssu72 and GSK3, GST pulldown assays were performed by incubating cellular extracts from differentiated 3T3-L1 cells with purified glutathione S-transferase (GST) or GST-fused Ssu72 (GST-Ssu72) proteins (Supplementary Fig

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have demonstrated that the hydrophobic loop of -SNAP is important not only for the coordinate binding of -SNAP to membranes but also for promoting or driving membrane fusion 12

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