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The levels of mRNA of target genes were normalized to GAPDH using a QuantStudio 7 Real-Time PCR system (Thermo Fisher, MA, USA)

Pretreatment of TC can preserve the activity of SOD, GPx, and CAT in the mitochondria as well as reduce the resultant oxidative damage ( Beta-asarone plays a neuroprotective role through the stabilization of the mitochondrial membrane potential to reduce Glu damage to neurons

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FDA drug safety communication: FDA warns about serious risks and death when combining opioid pain or cough medicines with benzodiazepines