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Description

Most people can benefit from a B12 injection, particularly those who live a busy life, are athletes, seniors, vegetarians, vegans, and those on prescription medications

This stiffness often lasts for about 30 minutes

Most protocols use once-daily injection for maintenance

Approximately 50 mg of frozen cecal content was added to 0.1 mm glass bead-containing tubes (PowerBead Tubes, Qiagen) prefilled with 300 l RLT buffer (RNeasy mini kit, Qiagen) supplemented with -mercaptoethanol (10 l/ml, Sigma-Aldrich) and 1 ml TRIzol (Invitrogen)