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N.ChenK.SomasundaramS

Levels decline significantly with age

The pharmacological effects of morroniside and loganin isolated from Liuweidihuang Wan, on MC3T3-E1 cells

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Individual response and healthcare provider recommendations determine the precise timing between injections, allowing for personalized treatment protocols that optimize results while maintaining safety

Cells were washed in PBS, resuspended in Thiazole Orange (BD Retic-Count, BD Bioscience) containing 4 M Hoechst 33342 (Invitrogen) for parasite RNA and DNA detection respectively ( 2 , 5 mM glucose, 32 mM HEPES pH7.4, 5 mM KCl, 1 mM MgSO4, 125 mM NaCl), resuspended in 0.3 M of intracellular calcium dye x-Rhod-1 AM (Invitrogen) and incubated in the dark at 37C for 30 min