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To test the effects of GHK and GHK-PEG on SH-SY5Yneuroblastomacell proliferation, cells were plated in 24 well plates and treated with GHK or PEGylated GHK at 10 and 20 nM for 24 hours and the effect of each treatment on cell proliferation was assessed using a hemocytometer

The addition of bacteriostatic preservatives such as benzyl alcohol became common practice during the twentieth century to allow multi-use solutions with reduced microbial contamination risk

As one of the most studied copper peptides in the scientific literature, GHK-Cu skin research has been particularly prolific, with numerous published studies exploring its role in extracellular matrix biology, collagen synthesis, and tissue remodeling processes

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Using it together with other ceramides, cholesterol, and linoleic acid have been shown to meaningfully improve hydration and reduce water loss