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The binding reactions were incubated and resolved by native polyacrylamide gel electrophoresis as described for 6-FAM-ARE 19 , except the binding reactions were resolved on a 6% (w/v) polyacrylamide/1X TB gel, and the gel was exposed to storage phosphor screen which was then scanned using an Amersham Typhoon Biomolecular Imager (Cytiva) on the phosphorimager setting

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