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The Ussing chamber technique is often utilised in animal studies where intestinal tissues may be obtained following sacrifice of the animals [41, 42] and in human studies of intestinal diseases where endoscopic biopsy samples may be obtained [43]

Moreover, increased homocysteine levels due to vitamin B12 deficiency could induce neurotoxicity caused by oxidative stress

Longevity pathways activated NAD+-dependent enzymes like SIRT1, tied to DNA repair and stress response, become more active

Phenytoin competes with valproate for binding to albumin, potentially increasing the free level of valproate

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