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For each reaction, 100 g of purified enzyme (diluted in 10 mM base buffer) in a total volume of 50 l is incubated with equal volume of substrate-liposome mix (prepared by mixing of 30 l 10 mM Dioleoylphosphatidylcholine, 30 l 10 mM Dioleoylphosphatidylglycerol, and 30 l 1 mM PLA 2 substrate) for 10 min at room temperature

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incubation of key CYP450 isoforms at 25C for six hours reduced their activity by about 90% (32)