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In: AggarwalBBSurhYJShishodiaS, editors

Ibrutinib Selects for Cells with Elevated Reactive Oxygen Species and Downregulated Phosphatases

To overcome this limitation, a new type of primer Editors was developed, and based on chimeric nCas9 protein fused to an M-MLV reverse transcriptase, a primer-editing guide RNA (pegRNA) designed to mediate site-specific nicking then serves as a template for RT (Anzalone et al., 2019)