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Samples were incubated on ice for 35 min, centrifuged for 20 min at 4 C at 12,000 rpm, and protein concentrations were quantified using the Pierce BCA Protein Assay Kit (ThermoFisher) according to the manufacturers microplate procedure protocol

Pregnant or Breastfeeding Women: The increased nutritional needs during pregnancy and breastfeeding may require B12 supplementation to support both mother and child

Human clinical trial data are absent

Daytime drowsiness: Some users experience grogginess if dosing is too high or poorly timed

Peters GJ, van der Wilt CL, van Moorsel CJ, Kroep JR, Bergman AM, Ackland SP

The figures below combine established pharmaceutical data and animal model extrapolation, labeled accordingly