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A common feature of plant subjected to environmental stress is the generation of reactive oxygen species (ROS) which are by-products of major cellular metabolic activities like plastidial, and mitochondrial electrons transfer chains, but also accumulate at excessive level upon stress conditions (Choudhury et al., 2017

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NNT knockdown shows limited ability to maintain NADPH levels and reduces tumorigenicity under oxidative stress conditions, such as that induced by anoikis, glucose deprivation in vitro, or impairs peritoneal dissemination and lung metastasis in vivo

As the N-terminal redox center (C154-C159) accepted electrons again, the protein was in the fully reduced state of EH 4

Next, we add loading buffer for WB analysis

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