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M., Palsson, B

Andrew: As people are hearing this, they're probably thinking, "Okay, this is what I call anec data or whatever." I don't have to remind people that you're a board-certified physician

FIGURE 7 Evidence L-citrulline administration as a means to promote NO production has received significant attention recently

DATS inhibits cell growth of human melanoma A375 cells and basal cell carcinoma (BCC) cells by increasing the levels of intracellular reactive oxygen species (ROS) and DNA damage and by inducing G2/M arrest, endoplasmic reticulum (ER) stress, and mitochondria-mediated apoptosis, including the caspase-dependent and -independent pathways

Unlike cytosolic thioredoxin that acts as an electron donor, most of the PDI family members have active site disulfides that are relatively unstable, making these enzymes poised to act as electron acceptors (Jeong et al

So thats how you can use GST pull-downs to investigate interactions with your protein of interest