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10.1136/bmj-2021-066452 BMJ

siRNA for xCT and siPORT NeoFX transfect agent were procured from Ambion, Texas, USA

Seeds were surface sterilized with bleach (3% sodium hypochlorite) for 8 min before being plated on half-strength sucrose-free Murashige and Skoog (1/2 MS0) media supplemented with Gamborgs vitamins (Caisson Laboratories, North Logan, UT), 0.5 mM MES (pH 5.7), and 0.8% (w/v) agar (Caisson Laboratories, North Logan, UT)

Soccio et al., 2010)

It demonstrates that the disruption of thiometabolism and disulfide stress play critical roles in CNS diseases, which are associated with the potential role of disulfidptosis

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