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Secondary symptoms include physical weakness, lumbar and knee soreness, frequent nocturnal urination, and diarrhea

An initial fungal cell density of OD 600 = 0.03 was inoculated into the 100 mL enriched chytrid growth medium in 250 mL flasks and cultured on a shaker incubator at 30 C, 150 rpm under continuous illumination (~ 10 mol m 2 s 1 )

doi:10.1016/j.actbio.2018.03.058 38

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However, diagnosing Wilson disease as the cause of acute liver failure can be challenging because elevated serum and urine copper levels are not specific to Wilson disease and can be seen in patients with acute liver failure from any cause

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