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Proteins were diluted with reaction buffer (60 mM HEPES pH 7.4, 25 mM NaCl, 25 mM KCl, 5% (v/v) glycerol, 10 mM MgCl 2 , 0.5 mM TCEP and 0.5 mg ml 1 BSA) supplemented with 5 mM ATP and 1 ATP regeneration mix (5 mM ATP, 0.03 mg ml 1 creatine kinase and 16 mM creatine phosphate)

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In parallel with analgesic tolerance, the ability of MOR to enhance [ 35 S]GTPS binding was reduced compared to naive animals (Bohn et al., 2010)

Tesamorelin is another GHRH analog with a specific approved-drug context, while GHRP-2, GHRP-6, Hexarelin, and MK-677 belong to the broader GH secretagogue landscape