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Oxidants induce Escherichia coli MarR glutathionylation in the presence of glutathione

It is involved in the tricarboxylic acid cycle, glucose metabolism, and can activate various enzymes to promote glycometabolism, lipid metabolism, and protein metabolism

Western blotting Tissue lysates were prepared with radioimmunoprecipitation assay buffer and separated on NuPAGE Bis-Tris Mini Gels (Life Technologies), followed by the transfer to polyvinylidene difluoride membrane (Millipore) following a standard protocol

Expect 25 to 40 percent improvement with optimal protocols

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The cysteine-glycine complex is then cleaved further into the respective amino acids then transported into the cell