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Summary: This long-term study evaluated the sustained efficacy and cardiovascular benefits of once-weekly semaglutide for up to four years

Preparation of cells for in vitro electrophysiology A single-cell suspension of dissociated glioma cells (see Cell culture) was plated at a density of 20,000 cells per well of a 24-well plate containing glass coverslips (Electron Microscopy Services) pre-coated with poly-l-lysine (Sigma) and 5 g ml 1 mouse laminin (Thermo Fisher Scientific)

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