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Western blotting analysis and enzyme-linked immunosorbent assay (ELISA) assay Whole liver lysates were prepared by sonication in HEPES lysis buffer (50 mM HEPES pH 7.5, 150 mM NaCl, 1 mM EDTA, 2.5 mM EGTA, 0.1% Tween 20, 10% glycerol, 0.1 mM Na orthovanadate, 1 mM NaF, 10 mM -glycerophosphate) containing protease inhibitor cocktail (EDTA-free Complete Mini, Roche, Meylan, France)

Des niveaux de glutathion abaisss et un ratio GSH/GSSG dsquilibr ont t observs dans les tissus crbraux de patients atteints de maladies d'Alzheimer et de Parkinson, dans le plasma de patients diabtiques de type 2, et dans les maladies cardiovasculaires